Johnson-Ajinwo, O, Richardson, A ORCID: https://orcid.org/0000-0003-1825-3375 and Li, W-W ORCID: https://orcid.org/0000-0002-3706-6068 (2019) Palmatine from Unexplored Rutidea parviflora Showed Cytotoxicity and Induction of Apoptosis in Human Ovarian Cancer Cells. Toxins, 11 (4). 237 - 237.

[img]
Preview
Text
W Li - Palmatine from unexplored rutidea parviflora showed....pdf - Published Version
Available under License Creative Commons Attribution.

Download (833kB) | Preview

Abstract

Ovarian cancer ranks amongst the deadliest cancers in the gynaecological category of cancers. This research work aims to evaluate in vitro anti-ovarian cancer activities and identify phytochemical constituents of a rarely explored plant speciesRutidea parviflora DC. The aqueous and organic extracts of the plant were evaluated for cytotoxicity using sulforhodamine B assay in four ovarian cancer cell lines and an immortalized human ovarian epithelial (HOE) cell line. The bioactive compounds were isolated and characterized by gas/liquid chromatography mass spectrometry and nuclear magnetic resonance spectroscopy. Caspase 3/7 activity assay, western blotting and flow cytometry were carried out to assess apoptotic effects of active compounds. The extracts/fractions of R. parviflora showed promising anti-ovarian cancer activities in ovarian cancer cell lines. A principal cytotoxic alkaloid was identified as palmatine whose IC50 was determined as 5.5-7.9 mu M. Palmatine was relatively selective towards cancer cells as it was less cytotoxic toward HOE cells, also demonstrating interestingly absence of cross-resistance in cisplatin-resistant A2780 cells. Palmatine further induced apoptosis by increasing caspase 3/7 activity, poly-ADP-ribose polymerase cleavage, and annexin V and propidium iodide staining in OVCAR-4 cancer cells. Our studies warranted further investigation of palmatine and R. parviflora extracts in preclinical models of ovarian cancer.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via MDPI AG at https://doi.org/10.3390/toxins11040237 - Please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: Ovarian cancer; Rutidea parviflora; Palmatine; Apoptosis
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > School of Pharmacy
Depositing User: Symplectic
Date Deposited: 30 Apr 2019 09:52
Last Modified: 28 May 2019 14:08
URI: http://eprints.keele.ac.uk/id/eprint/6244

Actions (login required)

View Item View Item