Allan, R, Sharples, AP ORCID: https://orcid.org/0000-0003-1526-9400, Cocks, M, Drust, B, Dutton, J, Dugdale, HF, Mawhinney, C, Clucas, A, Hawkins, W, Morton, JP and Gregson, W (2019) Low pre-exercise muscle glycogen availability offsets the effect of post exercise cold water immersion in augmenting PGC-1α gene expression. Physiological Reports, 7 (11). e14082.

[img]
Preview
Text
Allan_et_al-2019-Physiological_Reports (1).pdf - Published Version
Available under License Creative Commons Attribution.

Download (677kB) | Preview

Abstract

We assessed the effects of post‐exercise cold‐water immersion (CWI) in modulating PGC‐1α mRNA expression in response to exercise commenced with low muscle glycogen availability. In a randomized repeated‐measures design, nine recreationally active males completed an acute two‐legged high‐intensity cycling protocol (8 × 5 min at 82.5% peak power output) followed by 10 min of two‐legged post‐exercise CWI (8°C) or control conditions (CON). During each trial, one limb commenced exercise with low (LOW: <300 mmol·kg−1 dw) or very low (VLOW: <150 mmol·kg−1 dw) pre‐exercise glycogen concentration, achieved via completion of a one‐legged glycogen depletion protocol undertaken the evening prior. Exercise increased (P < 0.05) PGC‐1α mRNA at 3 h post‐exercise. Very low muscle glycogen attenuated the increase in PGC‐1α mRNA expression compared with the LOW limbs in both the control (CON VLOW 3.6‐fold vs. CON LOW 5.6‐fold: P = 0.023, ES 1.22 Large) and CWI conditions (CWI VLOW 2.4‐fold vs. CWI LOW 8.0 fold: P = 0.019, ES 1.43 Large). Furthermore, PGC‐1α mRNA expression in the CWI‐LOW trial was not significantly different to the CON LOW limb (P = 0.281, ES 0.67 Moderate). Data demonstrate that the previously reported effects of post‐exercise CWI on PGC‐1α mRNA expression (as regulated systemically via β‐adrenergic mediated cell signaling) are offset in those conditions in which local stressors (i.e., high‐intensity exercise and low muscle glycogen availability) have already sufficiently activated the AMPK‐PGC‐1α signaling axis. Additionally, data suggest that commencing exercise with very low muscle glycogen availability attenuates PGC‐1α signaling.

Item Type: Article
Additional Information: This is the final published version (version of record). It was first published online via Wiley at https://doi.org/10.14814/phy2.14082 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: Carbohydrate, cooling, skeletal muscle, training adaptation
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 06 Jun 2019 09:11
Last Modified: 06 Jun 2019 09:14
URI: http://eprints.keele.ac.uk/id/eprint/6449

Actions (login required)

View Item View Item