Foglia, F, Hazael, R, Meersman, F, Wilding, MC, Sakai, VG, Rogers, S, Bove, LE, Koza, MM, Moulin, M, Haertlein, M, Forsyth, VT and McMillan, PF (2019) In Vivo Water Dynamics in Shewanella oneidensis Bacteria at High Pressure. Scientific Reports, 9 (1). 8716 -?.

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Abstract

Following observations of survival of microbes and other life forms in deep subsurface environments it is necessary to understand their biological functioning under high pressure conditions. Key aspects of biochemical reactions and transport processes within cells are determined by the intracellular water dynamics. We studied water diffusion and rotational relaxation in live Shewanella oneidensis bacteria at pressures up to 500 MPa using quasi-elastic neutron scattering (QENS). The intracellular diffusion exhibits a significantly greater slowdown (by -10-30%) and an increase in rotational relaxation times (+10-40%) compared with water dynamics in the aqueous solutions used to resuspend the bacterial samples. Those results indicate both a pressure-induced viscosity increase and slowdown in ionic/macromolecular transport properties within the cells affecting the rates of metabolic and other biological processes. Our new data support emerging models for intracellular organisation with nanoscale water channels threading between macromolecular regions within a dynamically organized structure rather than a homogenous gel-like cytoplasm.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via Nature at http://doi.org/10.1038/s41598-019-44704-3 - please refer to any applicable terms of use of the publisher.
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Natural Sciences > School of Chemical and Physical Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 08 Jul 2019 11:39
Last Modified: 08 Jul 2019 11:39
URI: http://eprints.keele.ac.uk/id/eprint/6563

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