Don Paul, C, Kiss, C, Traore, DAK ORCID: https://orcid.org/0000-0003-1001-4716, Gong, L, Wilce, MCJ, Devenish, RJ, Bradbury, A and Prescott, M (2013) Phanta: a non-fluorescent photochromic acceptor for pcFRET. PLoS One, 8 (9). e75835 - ?.

[img]
Preview
Text
Phanta a non-fluorescent photochromic acceptor for pcFRET.pdf - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

We have developed an orange non-fluorescent photochromic protein (quantum yield, 0.003) we call Phanta that is useful as an acceptor in pcFRET applications. Phanta can be repeatedly inter-converted between the two absorbing states by alternate exposure to cyan and violet light. The absorption spectra of Phanta in one absorbing state shows excellent overlap with the emission spectra of a number of donor green fluorescent proteins including the commonly used EGFP. We show that the Phanta-EGFP FRET pair is suitable for monitoring the activation of caspase 3 in live cells using readily available instrumentation and a simple protocol that requires the acquisition of two donor emission images corresponding to Phanta in each of its photoswitched states. This the first report of a genetically encoded non-fluorescent acceptor for pcFRET.

Item Type: Article
Additional Information: © 2013 Don Paul et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Natural Sciences > School of Life Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 14 Aug 2019 11:22
Last Modified: 14 Aug 2019 11:22
URI: http://eprints.keele.ac.uk/id/eprint/6702

Actions (login required)

View Item View Item