Engineering CRISPR guide RNA riboswitches for in vivo applications

Galizi, Roberto

doi:10.1016/j.copbio.2018.08.007

Engineering CRISPR guide RNA riboswitches for in vivo applications

Galizi, Roberto

Authors

Roberto Galizi r.galizi@keele.ac.uk

Abstract

CRISPR-based genome editing provides a simple and scalable toolbox for a variety of therapeutic and biotechnology applications. Whilst the fundamental properties of CRISPR proved easily transferable from the native prokaryotic hosts to eukaryotic and multicellular organisms, the tight control of the CRISPR-editing activity remains a major challenge. Here we summarise recent developments of CRISPR and riboswitch technologies and recommend novel functionalised synthetic-gRNA (sgRNA) designs to achieve inducible and spatiotemporal regulation of CRISPR-based genetic editors in response to cellular or extracellular stimuli. We believe that future advances of these tools will have major implications for both basic and applied research, spanning from fundamental genetic studies and synthetic biology to genetic editing and gene therapy.

Journal Article Type	Article
Acceptance Date	Aug 16, 2018
Publication Date	Sep 25, 2018
Publicly Available Date	Jun 28, 2023
Journal	Current Opinion in Biotechnology
Print ISSN	0958-1669
Publisher	Elsevier
Pages	103 - 113
DOI	https://doi.org/10.1016/j.copbio.2018.08.007
Keywords	CRISPR, RNA, Riboswitches.
Publisher URL	https://www.sciencedirect.com/science/article/pii/S0958166918300569?via%3Dihub