Davies, DJ and Heylings, JR and McCarthy, TJ and Correa, CM (2015) Development of an in vitro model for studying the penetration of chemicals through compromised skin. Toxicol In Vitro, 29 (1). 176 - 181. ISSN 0887-2333

[img]
Preview
Text
J Heylings - Development of an in vitro model for studying the penetration of chemicals through compromised skin.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (560kB) | Preview

Abstract

The conventional safety approach that includes dermal absorption of pharmaceutical or consumer products uses models that are based on intact skin. However, when products are intended for application to skin with a less effective barrier, such as in new-born infants, or in cases where the skin is mildly damaged or diseased, there are instances where absorption through compromised skin is also important. A tape stripping procedure was investigated using dermatomed pig skin to assess if an in vitro model could replicate the typical changes in barrier function observed in humans with compromised skin. The relationship between Trans-Epidermal Water Loss (TEWL), Electrical Resistance (ER) and Tritiated Water Flux(TWF), markers of skin barrier function in OECD 428 studies was investigated. There was a step-wise reduction in ER from normal (control) skin following 5, 10, 15 or 20 tape strips. This was mirrored by increases in both TWF and TEWL. An in vitro experimental protocol using 5 tape strips, ER and dermatomed pig skin provided a rapid, robust and reproducible approach equivalent to the 3–4-fold increases in TEWL observed clinically in compromised skin.

Item Type: Article
Uncontrolled Keywords: dermatomed pig skin; electrical resistance; trans-epidermal water loss; tritiated water flux; tape stripping; in vitro percutaneous absorption
Related URLs:
Depositing User: Symplectic
Date Deposited: 13 Aug 2015 14:22
Last Modified: 22 Feb 2016 14:12
URI: http://eprints.keele.ac.uk/id/eprint/802

Actions (login required)

View Item View Item