Glossop, JR, Haworth, KE, Emes, RD, Nixon, NB, Packham, JC, Dawes, PT, Fryer, AA, Mattey, DL and Farrell, WE (2015) DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS. Epigenomics, 7 (4). 539 -551. ISSN 1750-192X

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Abstract

AIM: Alterations in DNA methylation contribute to the abnormal phenotype of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). We profiled genome-wide DNA methylation in these cells from synovial fluid, a more readily accessible source of disease-associated cells. PATIENTS & METHODS: Genome-wide DNA methylation was interrogated in fluid-derived FLS from five RA and six osteoarthritis patients using Human Methylation 450 Bead Chip and bisulfite pyrosequencing. RESULTS: Array analysis identified 328 CpGs, representing 195 genes, that were differentially methylated between RA and osteoarthritis fluid-derived FLS. Comparison with the genes identified in two independent studies of tissue-derived FLS revealed 73 genes in common (˜40%), of which 22 shared identity with both studies. Pyrosequencing confirmed altered methylation of these genes. CONCLUSION: Synovial fluid-derived RA FLS show methylation changes common with tissue-derived FLS, supporting the use of fluid-derived FLS for future investigations.

Item Type: Article
Uncontrolled Keywords: DNA methylation, epigenetics, fibroblast-like synoviocyte, osteoarthritis, rheumatoid arthritis, synovial fluid
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 17 Sep 2015 11:53
Last Modified: 18 Jul 2017 13:07
URI: http://eprints.keele.ac.uk/id/eprint/845

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