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DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS

Glossop, John R; Haworth, Kim E; Emes, Richard D; Nixon, Nicola B; Packham, Jon C; Dawes, Peter T; Fryer, Anthony A; Mattey, Derek L; Farrell, William E

Authors

John R Glossop

Kim E Haworth

Richard D Emes

Nicola B Nixon

Jon C Packham

Peter T Dawes

Derek L Mattey

William E Farrell



Abstract

AIM: Alterations in DNA methylation contribute to the abnormal phenotype of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). We profiled genome-wide DNA methylation in these cells from synovial fluid, a more readily accessible source of disease-associated cells. PATIENTS & METHODS: Genome-wide DNA methylation was interrogated in fluid-derived FLS from five RA and six osteoarthritis patients using Human Methylation 450 Bead Chip and bisulfite pyrosequencing. RESULTS: Array analysis identified 328 CpGs, representing 195 genes, that were differentially methylated between RA and osteoarthritis fluid-derived FLS. Comparison with the genes identified in two independent studies of tissue-derived FLS revealed 73 genes in common (˜40%), of which 22 shared identity with both studies. Pyrosequencing confirmed altered methylation of these genes. CONCLUSION: Synovial fluid-derived RA FLS show methylation changes common with tissue-derived FLS, supporting the use of fluid-derived FLS for future investigations.

Journal Article Type Article
Online Publication Date Jun 25, 2015
Publication Date Jun 1, 2015
Journal Epigenomics
Print ISSN 1750-1911
Publisher Future Medicine
Peer Reviewed Peer Reviewed
Volume 7
Issue 4
Pages 539 -551
DOI https://doi.org/10.2217/epi.15.15
Keywords DNA methylation, epigenetics, fibroblast-like synoviocyte, osteoarthritis, rheumatoid arthritis, synovial fluid
Publisher URL http://dx.doi.org/10.2217/epi.15.15
PMID 26111028