Hart, SR, Baker, PN, Kenny, L and Myers, JE (2015) Electron transfer dissociation of native peptides facilitates enhanced identification of urinary peptides. International Journal of Mass Spectrometry, 391. pp. 41-46. ISSN 1387-3806

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Abstract

Urine as a biofluid is commonly used in clinical diagnostics, including those performed during pregnancy. Urine is a rich source of polypeptides and polypeptidic protein degradation products, which have been filtered from blood plasma, thus urine has potential as a source for novel clinical diagnostics in disease. In this study, we examine the urinary peptidome from normal healthy women during pregnancy, and demonstrate ready observation of large polypeptide. We utilise the dissociation method, electron transfer dissociation (ETD) to increase the identification rate of the peptides present within these samples, as the polypeptide species observed in these samples are large and highly charged. An increase in the number of peptides whose identities could be ascribed using routine database searching methods was enabled via the use of ETD.

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via Elsevier at https://doi.org/10.1016/j.ijms.2015.08.025 Please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: peptidome, urine, electron transfer dissociation, pregnancy, non-tryptic peptides
Subjects: Q Science > QH Natural history
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Depositing User: Symplectic
Date Deposited: 09 Sep 2015 15:34
Last Modified: 13 Jun 2018 13:17
URI: http://eprints.keele.ac.uk/id/eprint/854

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