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Cross-Reactivity between Schistosoma mansoni Antigens and the Latex Allergen Hev b 7: Putative Implication of Cross-Reactive Carbohydrate Determinants (CCDs)

Liddell, S; Fuller, H; Stanley, RG; Schramm, G; Igetei, JE; Doenhoff, MJ; El-Faham, M

Authors

S Liddell

RG Stanley

G Schramm

JE Igetei

MJ Doenhoff

M El-Faham



Abstract

IgG antibodies produced by rabbits immunized against S. mansoni antigens cross-reacted with aqueous soluble constituents of a variety of allergens. The antibody cross-reactivity was largely sensitive to degradation by treatment of the target antigens with sodium meta-periodate, suggesting the cross-reactivity was due to carbohydrate determinants that were common to both the schistosome and the allergens (CCDs). The reaction between the rabbit antibodies and a 43 kDa molecule in a rubber latex extract was analysed further: tandem mass spectrometry identified the latex molecule as allergen Hev b 7. Rabbit anti-schistosome IgG antibodies purified by acid-elution from solid-phase latex Hev b 7 reacted with the S. mansoni egg antigens IPSE/alpha-1 and kappa-5 and cercarial antigens SPO-1 and a fatty acid-binding protein. Moreover, purified anti-S. mansoni egg, latex cross-reactive antibodies reacted with antigenic constituents of some fruits, a result of potential relevance to the latex-fruit syndrome of allergic reactions. We propose that IgG anti-schistosome antibodies that cross-react with allergens may be able to block IgE-induced allergic reactions and thus provide a possible explanation for the hygiene hypothesis.

Acceptance Date Jul 15, 2016
Publication Date Jul 28, 2016
Publicly Available Date Mar 29, 2024
Journal PLoS One
Print ISSN 1932-6203
Publisher Public Library of Science
DOI https://doi.org/10.1371/journal.pone.0159542
Keywords latex; antibodies; Schistosoma mansoni; Allergens; Immune Serum; Cross reactivity; Antigens; Allergies
Publisher URL http://dx.doi.org/10.1371/journal.pone.0159542