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SEC-SANS: size exclusion chromatography combined in situ with small-angle neutron scattering

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Abstract

The first implementation and use of an in situ size exclusion chromatography (SEC) system on a small-angle neutron scattering instrument (SANS) is described. The possibility of deploying such a system for biological solution scattering at the Institut Laue–Langevin (ILL) has arisen from the fact that current day SANS instruments at ILL now allow datasets to be acquired using small sample volumes with exposure times that are often shorter than a minute. This capability is of particular importance for the study of unstable biological macromolecules where aggregation or denaturation issues are a major problem. The first use of SEC-SANS on ILL's instrument D22 is described for a variety of proteins including one particularly aggregation-prone system.

Acceptance Date Oct 16, 2016
Publication Date Nov 2, 2016
Publicly Available Date Mar 29, 2024
Journal Journal of Applied Crystallography
Print ISSN 0021-8898
Publisher International Union of Crystallography
Pages 2015-2020
DOI https://doi.org/10.1107/S1600576716016514.
Keywords SEC-SANS; size exclusion chromatography; small-angle neutron scattering; in situ; sample environment
Publisher URL https://doi.org/10.1107/S1600576716016514

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