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Analysis of XFEL serial diffraction data from individual crystalline fibrils

Analysis of XFEL serial diffraction data from individual crystalline fibrils Thumbnail


Abstract

Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils; these patterns are weak and contain only a few reflections. Methods are developed for determining the orientation of patterns in reciprocal space and merging them in three dimensions. This allows the individual structure
amplitudes to be calculated, thus overcoming the limitations of orientation and cylindrical averaging in conventional fibre diffraction analysis. The advantages of this technique should allow structural studies of fibrous systems in biology that are inaccessible using existing techniques.

Acceptance Date Oct 4, 2017
Publication Date Nov 1, 2017
Publicly Available Date Mar 29, 2024
Journal IUCRJ
Publisher International Union of Crystallography
Pages 795 -811
DOI https://doi.org/10.1107/S2052252517014324
Keywords serial crystallography, coherent X-ray diffractive imaging (CXDI), single particles, molecular orientation determination, crystalline fibrils, amyloid
Publisher URL http://dx.doi.org/10.1107/S2052252517014324

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