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Dermal absorption of testosterone in human and pig skin in vitro

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Abstract

The OECD test guideline 428 for the assessment of dermal absorption in vitro has been in force for more than a decade. Various sectors of industry utilise the method for the registration of chemical products. These include the Agrochemical and Cosmetic sectors where the OECD test guideline and industry-specific guidance forms a key part of the human risk assessment process for new and existing products. This investigation has compared the dermal absorption characteristics of one of the OECD 428 reference chemicals, testosterone, in human and pig dermatomed skin. We used identical dosing and skin decontamination conditions for testosterone in Franz static diffusion cells. This included a full mass balance recovery of the dose applied and distribution of the compound in the different layers of the skin. Our investigation has shown that intact human skin provides a more effective barrier to the dermal absorption of testosterone compared with pig skin, when studied according to modem day in vitro dermal absorption guidance.

Acceptance Date Dec 20, 2017
Publication Date Apr 1, 2018
Publicly Available Date Mar 28, 2024
Journal Toxicology in Vitro
Print ISSN 0887-2333
Publisher Elsevier
Pages 71-77
DOI https://doi.org/10.1016/j.tiv.2017.12.014
Keywords Dermal absorption, Dermatomed skin, Electrical Resistance (ER), OECD 428, Tape stripping, In vitro percutaneous absorption
Publisher URL https://doi.org/10.1016/j.tiv.2017.12.014

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