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Development of an in vitro model for studying the penetration of chemicals through compromised skin.

Development of an in vitro model for studying the penetration of chemicals through compromised skin. Thumbnail


Abstract

The conventional safety approach that includes dermal absorption of pharmaceutical or consumer products uses models that are based on intact skin. However, when products are intended for application to skin with a less effective barrier, such as in new-born infants, or in cases where the skin is mildly damaged or diseased, there are instances where absorption through compromised skin is also important. A tape stripping procedure was investigated using dermatomed pig skin to assess if an in vitro model could replicate the typical changes in barrier function observed in humans with compromised skin. The relationship between Trans-Epidermal Water Loss (TEWL), Electrical Resistance (ER) and Tritiated Water Flux(TWF), markers of skin barrier function in OECD 428 studies was investigated. There was a step-wise reduction in ER from normal (control) skin following 5, 10, 15 or 20 tape strips. This was mirrored by increases in both TWF and TEWL. An in vitro experimental protocol using 5 tape strips, ER and dermatomed pig skin provided a rapid, robust and reproducible approach equivalent to the 3–4-fold increases in TEWL observed clinically in compromised skin.

Acceptance Date Sep 23, 2014
Publication Date Feb 1, 2015
Publicly Available Date Mar 28, 2024
Journal Toxicol In Vitro
Print ISSN 0887-2333
Publisher Elsevier
Pages 176 - 181
DOI https://doi.org/10.1016/j.tiv.2014.09.012
Keywords dermatomed pig skin; electrical resistance; trans-epidermal water loss; tritiated water flux; tape stripping; in vitro percutaneous absorption
Publisher URL http://www.sciencedirect.com/science/article/pii/S0887233314001854

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