Luo, L, Foster, NC, Man, KL, Brunet, M, Hoey, DA, Cox, SC, Kimber, SJ and El Haj, AJ ORCID: (2021) Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells. Biotechnology Journal.

Biotechnology Journal - 2021 - Luo - Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release.pdf - Accepted Version

Download (2MB) | Preview


Mechanical stimulation plays in an important role in regulating stem cell differentiation and their release of extracellular vesicles (EVs). In this study, we examined effects of low magnitude hydrostatic pressure (HP) on the chondrogenic differentiation and microvesicle release from human embryonic stem cells (hESCs) and human bone marrow stem cells (hBMSCs). hESCs were differentiated into chondroprogenitors and then embedded in fibrin gels and subjected to HP (270 kPa, 1 Hz, 5 days/week). hBMSC pellets were differentiated in chondrogenic media and subjected to the same regime. HP significantly enhanced ACAN expression in hESCs. It also led to a significant increase in DNA content, sGAG content and total sGAG/DNA level in hBMSCs. Furthermore, HP significantly increased microvesicle protein content released from both cell types. These results highlight the benefit of HP bioreactor in promoting chondrogenesis and EV production for cartilage tissue engineering.

Item Type: Article
Additional Information: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1002/biot.202100401.This article is protected by copyright. All rights reserved.
Uncontrolled Keywords: bone marrow stem cells; embryonic stem cells; extracellular vesicle; hydrostatic pressure; mechanical stimulation
Subjects: R Medicine > R Medicine (General)
R Medicine > RM Therapeutics. Pharmacology
Depositing User: Symplectic
Date Deposited: 11 Jan 2022 16:30
Last Modified: 22 Feb 2022 09:13

Actions (login required)

View Item View Item