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Differences in magnetic particle uptake by CNS neuroglial subclasses: implications for neural tissue engineering

Jenkins, SI; Pickard, MR; Furness, DN; Yiu, HH; Chari, DM

Differences in magnetic particle uptake by CNS neuroglial subclasses: implications for neural tissue engineering Thumbnail


Authors

MR Pickard

DN Furness

HH Yiu



Abstract

AIM: To analyze magnetic particle uptake and intracellular processing by the four main non-neuronal subclasses of the CNS: oligodendrocyte precursor cells; oligodendrocytes; astrocytes; and microglia. MATERIALS & METHODS: Magnetic particle uptake and processing were studied in rat oligodendrocyte precursor cells and oligodendrocytes using fluorescence and transmission electron microscopy, and the results collated with previous data from rat microglia and astrocyte studies. All cells were derived from primary mixed glial cultures. RESULTS: Significant intercellular differences were observed between glial subtypes: microglia demonstrate the most rapid/extensive particle uptake, followed by astrocytes, with oligodendrocyte precursor cells and oligodendrocytes showing significantly lower uptake. Ultrastructural analyses suggest that magnetic particles are extensively degraded in microglia, but relatively stable in other cells. CONCLUSION: Intercellular differences in particle uptake and handling exist between the major neuroglial subtypes. This has important implications for the utility of the magnetic particle platform for neurobiological applications including genetic modification, transplant cell labeling and biomolecule delivery to mixed CNS cell populations.

Acceptance Date Nov 22, 2012
Publication Date Jun 1, 2013
Publicly Available Date Mar 29, 2024
Journal Nanomedicine
Print ISSN 1743-5889
Publisher Future Medicine
Pages 951 -968
DOI https://doi.org/10.2217/nnm.12.145
Keywords endocytosis, magnetic particle, neuroglia, oligodendrocyte precursor cell, uptake
Publisher URL http://dx.doi.org/10.2217/nnm.12.145

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