Drizou, F, Graham, NS, Bruce, TJA and Ray, RV (2017) Development of high-throughput methods to screen disease caused by Rhizoctonia solani AG 2-1 in oilseed rape. Plant Methods, 13. 45 -?.

Development of high-throughput methods to screen disease caused by Rhizoctonia solani AG 2-1 in oilseed rape.pdf - Published Version
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BACKGROUND: Rhizoctonia solani (Kühn) is a soil-borne, necrotrophic fungus causing damping off, root rot and stem canker in many cultivated plants worldwide. Oilseed rape (OSR, Brassica napus) is the primary host for anastomosis group (AG) 2-1 of R. solani causing pre- and post-emergence damping-off resulting in death of seedlings and impaired crop establishment. Presently, there are no known resistant OSR genotypes and the main methods for disease control are fungicide seed treatments and cultural practices. The identification of sources of resistance for crop breeding is essential for sustainable management of the disease. However, a high-throughput, reliable screening method for resistance traits is required. The aim of this work was to develop a low cost, rapid screening method for disease phenotyping and identification of resistance traits. RESULTS: Four growth systems were developed and tested: (1) nutrient media plates, (2) compost trays, (3) light expanded clay aggregate (LECA) trays, and (4) a hydroponic pouch and wick system. Seedlings were inoculated with virulent AG 2-1 to cause damping-off disease and grown for a period of 4-10 days. Visual disease assessments were carried out or disease was estimated through image analysis using ImageJ. CONCLUSION: Inoculation of LECA was the most suitable method for phenotyping disease caused by R. solani AG 2-1 as it enabled the detection of differences in disease severity among OSR genotypes within a short time period whilst allowing measurements to be conducted on whole plants. This system is expected to facilitate identification of resistant germplasm.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via BioMed Central at http://dx.doi.org/10.1186/s13007-017-0195-1 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: rhizoctonia solani, oilseed rape, high-throughput phenotyping, disease, plant characteristics
Subjects: Q Science > QH Natural history
Divisions: Faculty of Natural Sciences > School of Life Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 27 Sep 2017 13:20
Last Modified: 27 Sep 2017 13:20
URI: https://eprints.keele.ac.uk/id/eprint/4054

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