Watkinson
Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase
Watkinson
Authors
Abstract
Peptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthritis and osteoarthritis. The detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a protease biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film and demonstrated for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals, and their degradation in the presence of collagenase was monitored using a quartz crystal microbalance (QCM). The biosensor was shown to respond to concentrations between 2 and 2000 nM in less than 10 min with a lower detection limit of 2 nM.
Acceptance Date | Apr 2, 2019 |
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Publication Date | Apr 8, 2019 |
Publicly Available Date | Mar 28, 2024 |
Journal | Sensors |
Publisher | MDPI |
DOI | https://doi.org/10.3390/s19071677 |
Keywords | protease biosensor; collagenase; poly(ethylene glycol) norbornene; QCM; hydrogel degradation; peptide cross-links; click chemistry |
Publisher URL | https://doi.org/10.3390/s19071677 |
Files
sensors-19-01677-v2.pdf
(2.3 Mb)
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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