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Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase

Watkinson

Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase Thumbnail


Authors

Watkinson



Abstract

Peptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthritis and osteoarthritis. The detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a protease biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film and demonstrated for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals, and their degradation in the presence of collagenase was monitored using a quartz crystal microbalance (QCM). The biosensor was shown to respond to concentrations between 2 and 2000 nM in less than 10 min with a lower detection limit of 2 nM.

Acceptance Date Apr 2, 2019
Publication Date Apr 8, 2019
Publicly Available Date Mar 28, 2024
Journal Sensors
Publisher MDPI
DOI https://doi.org/10.3390/s19071677
Keywords protease biosensor; collagenase; poly(ethylene glycol) norbornene; QCM; hydrogel degradation; peptide cross-links; click chemistry
Publisher URL https://doi.org/10.3390/s19071677

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