LCGF Palhares
A Further Unique Chondroitin Sulfate from the shrimp Litopenaeus vannamei with Antithrombin Activity that Modulates Acute Inflammation
Palhares, LCGF; Brito, AS; Andrade De Lima, M; Nader, HB; London, JA; Barsukov, IL; Andrade, GPV; Yates, EA; Chavante, SF
Authors
AS Brito
Marcelo Andrade De Lima m.andrade.de.lima@keele.ac.uk
HB Nader
JA London
IL Barsukov
GPV Andrade
EA Yates
SF Chavante
Abstract
The detailed structure of a further Chondroitin Sulfate from Litopenaeus vannamei shrimp (sCS) is described. The backbone structure was established by 1H/13C NMR, which identified 3-O-sulfated GlcA, 4-O-sulfated GalNAc, 6-O-sulfated GalNAc, and 4,6-di-O-sulfated GalNAc residues. GlcA is linked to GalNAc 4,6 di S and GlcA 3S is linked to GalNAc 4S, GalNAc 4,6 di-S and GalNAc6S residues. The anticoagulant properties of this sCS were evaluated by activated partial thromboplastin time, anti-IIa, anti-Xa and anti-heparin cofactor II-mediated activities, and sCS failed to stabilise antithrombin in a fluoresence shift assay. The anti-inflammatory effect of sCS was explored using a model of acute peritonitis, followed by leukocyte count and measurement of the cytokines, IL-1ß, IL-6 and TNF-a. The compound showed low clotting effects, but high anti-IIa activity and HCII-mediated thrombin inhibition. Its anti-inflammatory effect was shown by leukocyte recruitment inhibition and a decrease in pro-inflammatory cytokine levels. Although the biological role of sCS remains unknown, its properties indicate that it is suitable for studies of multi-potent molecules obtained from natural sources.
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 25, 2019 |
Publication Date | Oct 15, 2019 |
Publicly Available Date | Mar 29, 2024 |
Journal | Carbohydrate Polymers |
Print ISSN | 0144-8617 |
Publisher | Elsevier |
Pages | 115031--115031 |
DOI | https://doi.org/10.1016/j.carbpol.2019.115031 |
Keywords | Chondroitin sulfate; Inflammation; Shrimp; Thrombin. |
Publisher URL | http://www.sciencedirect.com/science/article/pii/S0144861719306988 |
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https://creativecommons.org/licenses/by-nc-nd/4.0/
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