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A Further Unique Chondroitin Sulfate from the shrimp Litopenaeus vannamei with Antithrombin Activity that Modulates Acute Inflammation

Palhares, LCGF; Brito, AS; Andrade De Lima, M; Nader, HB; London, JA; Barsukov, IL; Andrade, GPV; Yates, EA; Chavante, SF

A Further Unique Chondroitin Sulfate from the shrimp Litopenaeus vannamei with Antithrombin Activity that Modulates Acute Inflammation Thumbnail


Authors

LCGF Palhares

AS Brito

HB Nader

JA London

IL Barsukov

GPV Andrade

EA Yates

SF Chavante



Abstract

The detailed structure of a further Chondroitin Sulfate from Litopenaeus vannamei shrimp (sCS) is described. The backbone structure was established by 1H/13C NMR, which identified 3-O-sulfated GlcA, 4-O-sulfated GalNAc, 6-O-sulfated GalNAc, and 4,6-di-O-sulfated GalNAc residues. GlcA is linked to GalNAc 4,6 di S and GlcA 3S is linked to GalNAc 4S, GalNAc 4,6 di-S and GalNAc6S residues. The anticoagulant properties of this sCS were evaluated by activated partial thromboplastin time, anti-IIa, anti-Xa and anti-heparin cofactor II-mediated activities, and sCS failed to stabilise antithrombin in a fluoresence shift assay. The anti-inflammatory effect of sCS was explored using a model of acute peritonitis, followed by leukocyte count and measurement of the cytokines, IL-1ß, IL-6 and TNF-a. The compound showed low clotting effects, but high anti-IIa activity and HCII-mediated thrombin inhibition. Its anti-inflammatory effect was shown by leukocyte recruitment inhibition and a decrease in pro-inflammatory cytokine levels. Although the biological role of sCS remains unknown, its properties indicate that it is suitable for studies of multi-potent molecules obtained from natural sources.

Journal Article Type Article
Acceptance Date Jun 25, 2019
Publication Date Oct 15, 2019
Publicly Available Date Mar 29, 2024
Journal Carbohydrate Polymers
Print ISSN 0144-8617
Publisher Elsevier
Pages 115031--115031
DOI https://doi.org/10.1016/j.carbpol.2019.115031
Keywords Chondroitin sulfate; Inflammation; Shrimp; Thrombin.
Publisher URL http://www.sciencedirect.com/science/article/pii/S0144861719306988

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