An alternative modular 'click-SNAr-click' approach to develop subcellular localised fluorescent probes to image mobile Zn2+

Watkinson, Mike

doi:10.1039/c9ob01855g

An alternative modular 'click-SNAr-click' approach to develop subcellular localised fluorescent probes to image mobile Zn2+

Watkinson, Mike

Authors

Mike Watkinson

Abstract

Zn2+ is involved in a number of biological processes and its wide-ranging roles at the subcellular level, especially in specific organelles, have not yet been fully established due to a lack of tools to image it effectively. We report a new and efficient modular double ‘click’ approach towards a range of sub-cellular localised probes for mobile zinc. Through this methodology, endoplasmic reticulum, mitochondria and lysosome localised probes were successfully prepared which show good fluorescence responses to mobile Zn2+ in vitro and in cellulo whilst a non-targeting probe was synthesized as a control. The methodology appears to have wide-utility for the generation of sub-cellular localised probes by incorporating specific organelle targeting vectors for mobile Zn2+ imaging.

Journal Article Type	Article
Acceptance Date	Oct 8, 2019
Publication Date	Dec 21, 2019
Publicly Available Date	May 26, 2023
Journal	ORGANIC & BIOMOLECULAR CHEMISTRY
Print ISSN	1477-0520
Publisher	Royal Society of Chemistry
Volume	17
Issue	47
Pages	10013-10019
DOI	https://doi.org/10.1039/c9ob01855g
Publisher URL	http://doi.org/10.1039/C9OB01855G