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Bhusal, RP, Jiao, W, Kwai, BXC, Reynisson, J ORCID: https://orcid.org/0000-0003-4174-9512, Collins, AJ, Sperry, J, Bashiri, G and Leung, IKH
(2019)
Acetyl-CoA-mediated activation of Mycobacterium tuberculosis isocitrate lyase 2.
Nature Communications, 10 (1).
4639 - ?.
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s41467-019-12614-7.pdf - Published Version Available under License Creative Commons Attribution Non-commercial. Download (1MB) | Preview |
Abstract
Isocitrate lyase is important for lipid utilisation by Mycobacterium tuberculosis but its ICL2 isoform is poorly understood. Here we report that binding of the lipid metabolites acetyl-CoA or propionyl-CoA to ICL2 induces a striking structural rearrangement, substantially increasing isocitrate lyase and methylisocitrate lyase activities. Thus, ICL2 plays a pivotal role regulating carbon flux between the tricarboxylic acid (TCA) cycle, glyoxylate shunt and methylcitrate cycle at high lipid concentrations, a mechanism essential for bacterial growth and virulence.
Item Type: | Article |
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Additional Information: | This is the final published version of the article (version of record). It first appeared online via Springer Nature at https://doi.org/10.1038/s41467-019-12614-7 - please refer to any applicable terms of use of the publisher. |
Uncontrolled Keywords: | Biochemistry, Enzyme mechanisms, SAXS, X-ray crystallography |
Subjects: | R Medicine > RS Pharmacy and materia medica |
Divisions: | Faculty of Medicine and Health Sciences > School of Pharmacy and Bioengineering |
Related URLs: | |
Depositing User: | Symplectic |
Date Deposited: | 28 Oct 2019 12:13 |
Last Modified: | 28 Oct 2019 12:19 |
URI: | https://eprints.keele.ac.uk/id/eprint/7095 |
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