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Dynamics of cell wall assembly during early embryogenesis in the brown alga Fucus.

Torode, T; Siméon, A; Marcus, SE; Jam, M; Le Moigne, M-A; Duffieux, D; Knox, JP; Hervé, C

Dynamics of cell wall assembly during early embryogenesis in the brown alga Fucus. Thumbnail


Authors

A Siméon

SE Marcus

M Jam

M-A Le Moigne

D Duffieux

JP Knox

C Hervé



Abstract

Zygotes from Fucus species have been used extensively to study cell polarization and rhizoid outgrowth, and in this model system cell wall deposition aligns with the establishment of polarity. Monoclonal antibodies are essential tools for the in situ analysis of cell wall glycans, and here we report the characteristics of six monoclonal antibodies to alginates (BAM6-BAM11). The use of these, in conjunction with monoclonal antibodies to brown algal sulfated fucans, has enabled the study of the developmental dynamics of the Fucus zygote cell walls. Young zygotes are spherical and all alginate epitopes are deposited uniformly following cellulose deposition. At germination, sulfated fucans are secreted in the growing rhizoid wall. The redistribution of cell wall epitopes was investigated during treatments that cause reorientation of the growth axis (change in light direction) or disrupt rhizoid development (arabinogalactan-protein-reactive Yariv reagent). Alginate modeling was drastically impaired in the latter, and both treatments cause a redistribution of highly sulfated fucan epitopes. The dynamics of cell wall glycans in this system have been visualized in situ for the first time, leading to an enhanced understanding of the early developmental mechanisms of Fucus species. These sets of monoclonal antibodies significantly extend the available molecular tools for brown algal cell wall studies.

Acceptance Date Oct 6, 2016
Publication Date Oct 6, 2016
Publicly Available Date Mar 28, 2024
Journal Journal of Experiment Botany
Print ISSN 0022-0957
Publisher Oxford University Press
Pages 6089 - 6100
DOI https://doi.org/10.1093/jxb/erw369
Keywords cell wall, embryogenesis, fucus, brown alga.
Publisher URL https://academic.oup.com/jxb/article/67/21/6089/2738945

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