Everett, J ORCID: https://orcid.org/0000-0002-1864-9700, Céspedes, E, Shelford, LR, Exley, C ORCID: https://orcid.org/0000-0002-5116-7607, Collingwood, JF, Dobson, J, van der Laan, G, Jenkins, CA, Arenholz, E and Telling, ND ORCID: https://orcid.org/0000-0002-2683-5546 (2014) Ferrous iron formation following the co-aggregation of ferric iron and the Alzheimer's disease peptide β-amyloid (1-42). Journal of the Royal Society Interface, 11 (95). 20140165 - ?.

[img]
Preview
Text
Ferrous iron formation following the co-aggregation of ferric iron and the Alzheimers disease peptide β-amyloid (1-42).pdf - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

For decades, a link between increased levels of iron and areas of Alzheimer's disease (AD) pathology has been recognized, including AD lesions comprised of the peptide β-amyloid (Aβ). Despite many observations of this association, the relationship between Aβ and iron is poorly understood. Using X-ray microspectroscopy, X-ray absorption spectroscopy, electron microscopy and spectrophotometric iron(II) quantification techniques, we examine the interaction between Aβ(1-42) and synthetic iron(III), reminiscent of ferric iron stores in the brain. We report Aβ to be capable of accumulating iron(III) within amyloid aggregates, with this process resulting in Aβ-mediated reduction of iron(III) to a redox-active iron(II) phase. Additionally, we show that the presence of aluminium increases the reductive capacity of Aβ, enabling the redox cycling of the iron. These results demonstrate the ability of Aβ to accumulate iron, offering an explanation for previously observed local increases in iron concentration associated with AD lesions. Furthermore, the ability of iron to form redox-active iron phases from ferric precursors provides an origin both for the redox-active iron previously witnessed in AD tissue, and the increased levels of oxidative stress characteristic of AD. These interactions between Aβ and iron deliver valuable insights into the process of AD progression, which may ultimately provide targets for disease therapies.

Item Type: Article
Additional Information: © 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.
Uncontrolled Keywords: wustite; redox; Alzheimer's disease; X-ray absorption
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > School of Pharmacy and Bioengineering
Related URLs:
Depositing User: Symplectic
Date Deposited: 07 Jul 2020 14:04
Last Modified: 07 Jul 2020 14:04
URI: https://eprints.keele.ac.uk/id/eprint/8348

Actions (login required)

View Item View Item