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Electron transfer dissociation of native peptides facilitates enhanced identification of urinary peptides

Hart, Sarah R.; Kenny, Louise C.; Myers, Jenny E.; Baker, Philip N.

Electron transfer dissociation of native peptides facilitates enhanced identification of urinary peptides Thumbnail


Authors

Louise C. Kenny

Jenny E. Myers

Philip N. Baker



Abstract

Urine as a biofluid is commonly used in clinical diagnostics, including those performed during pregnancy. Urine is a rich source of polypeptides and polypeptidic protein degradation products, which have been filtered from blood plasma, thus urine has potential as a source for novel clinical diagnostics in disease. In this study, we examine the urinary peptidome from normal healthy women during pregnancy, and demonstrate ready observation of large polypeptide. We utilise the dissociation method, electron transfer dissociation (ETD) to increase the identification rate of the peptides present within these samples, as the polypeptide species observed in these samples are large and highly charged. An increase in the number of peptides whose identities could be ascribed using routine database searching methods was enabled via the use of ETD.

Journal Article Type Article
Acceptance Date Aug 25, 2015
Online Publication Date Sep 3, 2015
Publication Date Nov 30, 2015
Publicly Available Date Mar 29, 2024
Journal International Journal of Mass Spectrometry
Print ISSN 1387-3806
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 391
Pages 41-46
DOI https://doi.org/10.1016/j.ijms.2015.08.025
Keywords peptidome, urine, electron transfer dissociation, pregnancy, non-tryptic peptides
Publisher URL https://doi.org/10.1016/j.ijms.2015.08.025

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