Sharma, N, Langley, RJ, Eurtivong, C, Leung, E, Dixon, RJ, Paulin, EK, Rees, SWP, Pilkington, LI, Barker, D, Reynisson, J ORCID: https://orcid.org/0000-0003-4174-9512 and Leung, IKH (2021) An optimised MALDI-TOF assay for phosphatidylcholine-specific phospholipase C. Analytical Methods.

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Abstract

The Bacillus cereus phosphatidylcholine-specific phospholipase C (PC-PLCBc) is an enzyme that catalyses the hydrolysis of phosphatidylcholines into phosphocholine and 1,2-diacylglycerols. PC-PLCBc has found applications in both the food industry and in medicinal chemistry. Herein, we report our work in the development and optimisation of a matrix assisted laser desorption ionisation time-of-flight (MALDI-TOF) mass spectrometry-based assay to monitor PC-PLCBc activity. The use of one-phase and two-phase reaction systems to assess the inhibition of PC-PLCBc with different structural classes of inhibitors was compared. We also highlighted the advantage of our assay over the commonly used commercially available Amplex Red assay. This method will also be applicable to work on the activity and inhibition of other phospholipases.

Item Type: Article
Additional Information: The final version of this accepted manuscript and all relevant information can be found online at; https://pubs.rsc.org/en/content/articlelanding/2021/AY/D0AY02208J#!divAbstract
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry > QD415 Biochemistry
Divisions: Faculty of Medicine and Health Sciences > School of Pharmacy and Bioengineering
Related URLs:
Depositing User: Symplectic
Date Deposited: 05 Feb 2021 09:28
Last Modified: 05 Feb 2021 09:28
URI: https://eprints.keele.ac.uk/id/eprint/9132

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