Misirli, G ORCID: https://orcid.org/0000-0002-2454-7188, Yang, B, James, K and Wipat, A Virtual Parts Repository 2: Model-driven design of genetic regulatory circuits. bioRxiv. (Unpublished)

[img]
Preview
Text
2021.04.11.439316v1.full.pdf - Draft Version

Download (3MB) | Preview

Abstract

<jats:title>Abstract</jats:title><jats:p>Engineering genetic regulatory circuits is key to the creation of biological applications that are responsive to environmental changes. Computational models can assist in understanding especially large and complex circuits where manual analysis is infeasible, permitting a model-driven design process. However, there are still few tools that offer the ability to simulate the system under design. One of the reasons for this is the lack of accessible model repositories or libraries that cater for the modular composition of models of synthetic systems that do not yet exist in nature. Here, we present the Virtual Parts Repository 2, a resource to facilitate the model-driven design of genetic regulatory circuits, which provides reusable, modular and composable models. The repository is service-oriented and can be utilized by design tools in computational workflows. Designs provided in Synthetic Biology Open Language documents are used to derive system-scale and hierarchical Systems Biology Markup Language models. We also present a rule-based modeling abstraction based on reaction networks to facilitate scalable and modular modeling of complex and large designs. This modeling abstraction incorporates design patterns such as roadblocking, distributed deployment of genetic circuits using plasmids and cellular resource dependency. The computational resources and the modeling abstraction presented in this paper allow computational design tools to take advantage of computational simulations and ultimately help facilitate more predictable applications.</jats:p><jats:sec><jats:title>Graphical TOC Entry</jats:title><jats:fig id="ufig1" position="float" fig-type="figure" orientation="portrait"><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="439316v1_ufig1" position="float" orientation="portrait" /></jats:fig></jats:sec>

Item Type: Article
Additional Information: The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Subjects: Q Science > Q Science (General)
T Technology > T Technology (General)
T Technology > TA Engineering (General). Civil engineering (General)
Divisions: Faculty of Natural Sciences > School of Computing and Mathematics
Related URLs:
Depositing User: Symplectic
Date Deposited: 30 Apr 2021 11:47
Last Modified: 30 Apr 2021 11:47
URI: https://eprints.keele.ac.uk/id/eprint/9469

Actions (login required)

View Item View Item