Fang, L, Crespo-Otero, R, Jones, CR and Watkinson, M ORCID: https://orcid.org/0000-0003-1877-5667 (2021) Protect to detect: A Golgi apparatus targeted probe to image mobile zinc through the use of a lipophilic cell-labile protecting group strategy. Sensors and Actuators B: Chemical, 338.

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Abstract

The Golgi apparatus requires zinc for its normal function, but the role it plays in these processes at the sub-cellular level is not well-understood due to the lack of appropriate tools to image it. Herein, a small molecule Golgi apparatus targeted probe was developed to image mobile Zn2+. A trityl group was used to protect a Golgi apparatus targeting cysteine residue to increase cell membrane permeability, which was then removed within 24 h, revealing the free cysteine targeting motif to anchor the probe to the Golgi apparatus. The probe shows good photophysical properties, good selectivity and Zn2+ response over a wide range of pH as well as low cellular toxicity. The probe was shown to be capable of targeting the Golgi apparatus and responding to Zn2+ in a number of different cell lines and was also applied to monitor the change of concentration of mobile Zn2+ in the Golgi apparatus in response to oxidative stress.

Item Type: Article
Additional Information: The final version of this accepted manuscript and all relevant information related to it, including copyrights, can be found online at; https://www.sciencedirect.com/science/article/pii/S0925400521004196?via%3Dihub
Uncontrolled Keywords: Golgi apparatus targeting; Fluorescent probe; Zinc imaging; Click chemistry; Oxidative stress
Subjects: ?? Golgi apparatus targeting ??
?? Fluorescent probe ??
?? Zinc imaging ??
?? Click chemistry ??
?? Oxidative stress ??
Divisions: Faculty of Natural Sciences > School of Chemical and Physical Sciences
Depositing User: Symplectic
Date Deposited: 14 May 2021 07:49
Last Modified: 14 May 2021 07:49
URI: https://eprints.keele.ac.uk/id/eprint/9574

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