Gajdos, L, Blakeley, MP, Kumar, A, Wimmerová, M, Haertlein, M, Forsyth, VT ORCID: https://orcid.org/0000-0003-0380-3477, Imberty, A and Devos, JM (2021) Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions. Structure.

[img]
Preview
Text
Visualization of hydrogen atoms.pdf - Published Version

Download (5MB) | Preview

Abstract

Carbohydrate-binding proteins from pathogenic bacteria and fungi have been shown to be implicated in various pathological processes, where they interact with glycans present on the surface of the host cells. These interactions are part of the initial processes of infection of the host and are very important to study at the atomic level. Here, we report the room temperature neutron structures of PLL lectin from Photorhabdus laumondii in its apo form and in complex with deuterated L-fucose, which is, to our knowledge, the first neutron structure of a carbohydrate-binding protein in complex with a fully deuterated carbohydrate ligand. A detailed structural analysis of the lectin-carbohydrate interactions provides information on the hydrogen bond network, the role of water molecules, and the extent of the CH-π stacking interactions between fucose and the aromatic amino acids in the binding site.

Item Type: Article
Additional Information: Gajdos et al., Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of pro-tein-carbohydrate interactions, Structure (2021), https://doi.org/10.1016/j.str.2021.03.003 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QD Chemistry > QD415 Biochemistry
Related URLs:
Depositing User: Symplectic
Date Deposited: 04 Jun 2021 10:42
Last Modified: 04 Jun 2021 10:42
URI: https://eprints.keele.ac.uk/id/eprint/9661

Actions (login required)

View Item View Item