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Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells

Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells Thumbnail


Abstract

AbstractMechanical stimulation plays in an important role in regulating stem cell differentiation and their release of extracellular vesicles (EVs). In this study, effects of low magnitude hydrostatic pressure (HP) on the chondrogenic differentiation and microvesicle release from human embryonic stem cells (hESCs) and human bone marrow stem cells (hBMSCs) are examined. hESCs were differentiated into chondroprogenitors and then embedded in fibrin gels and subjected to HP (270 kPa, 1 Hz, 5 days per week). hBMSC pellets were differentiated in chondrogenic media and subjected to the same regime. HP significantly enhanced ACAN expression in hESCs. It also led to a significant increase in DNA content, sGAG content and total sGAG/DNA level in hBMSCs. Furthermore, HP significantly increased microvesicle protein content released from both cell types. These results highlight the benefit of HP bioreactor in promoting chondrogenesis and EV production for cartilage tissue engineering.

Journal Article Type Article
Acceptance Date Dec 14, 2021
Online Publication Date Jan 17, 2022
Publication Date Dec 18, 2021
Publicly Available Date Mar 29, 2024
Journal Biotechnology Journal
Print ISSN 1860-6768
Publisher Wiley-VCH Verlag
Volume 17
Issue 4
DOI https://doi.org/10.1002/biot.202100401
Keywords bone marrow stem cells; embryonic stem cells; extracellular vesicle; hydrostatic pressure; mechanical stimulation
Publisher URL https://onlinelibrary.wiley.com/doi/10.1002/biot.202100401

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