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UC.183, UC.110, and UC.84 Ultra-Conserved RNAs Are Mutually Exclusive with miR-221 and Are Engaged in the Cell Cycle Circuitry in Breast Cancer Cell Lines.

Corrà, Fabio; Crudele, Francesca; Baldassari, Federica; Bianchi, Nicoletta; Galasso, Marco; Minotti, Linda; Agnoletto, Chiara; Di Leva, Gianpiero; Brugnoli, Federica; Reali, Eva; Bertagnolo, Valeria; Vecchione, Andrea; Volinia, Stefano

UC.183, UC.110, and UC.84 Ultra-Conserved RNAs Are Mutually Exclusive with miR-221 and Are Engaged in the Cell Cycle Circuitry in Breast Cancer Cell Lines. Thumbnail


Authors

Fabio Corrà

Francesca Crudele

Federica Baldassari

Nicoletta Bianchi

Marco Galasso

Linda Minotti

Chiara Agnoletto

Federica Brugnoli

Eva Reali

Valeria Bertagnolo

Andrea Vecchione

Stefano Volinia



Abstract

In the human genome, there are about 600 ultra-conserved regions (UCRs), long DNA sequences extremely conserved in vertebrates. We performed a large-scale study to quantify transcribed UCR (T-UCR) and miRNA levels in over 6000 cancer and normal tissue samples to find possible correlation between these kinds of regulatory molecules. Our analysis evidenced several non-coding RNAs showing negative co-regulation with miRNAs; among them, we focused on miR-221 to investigate any relationship with its pivotal role in the cell cycle. We have chosen breast cancer as model, using two cell lines with different phenotypes to carry out in vitro treatments with siRNAs against T-UCRs. Our results demonstrate that the expression of uc.183, uc.110, and uc.84 T-UCRs is mutually exclusive with miR-221 and is engaged in the regulation of CDKN1B expression. In addition, tests with a set of anticancer drugs, including BYL719, AZD5363, AZD8055, AZD7762, and XL765, revealed the modulation of specific T-UCRs without alteration of miR-221 levels.

Journal Article Type Article
Acceptance Date Dec 8, 2021
Publication Date Dec 13, 2021
Publicly Available Date Mar 28, 2024
Journal Genes (Basel)
Print ISSN 2073-4425
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 12
Issue 12
Article Number 1978
DOI https://doi.org/10.3390/genes12121978
Publisher URL https://www.mdpi.com/2073-4425/12/12/1978

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