Matthews, H, McDonald, J, Totanes, FIG and Merrick, CJ (2022) Dynamics of DNA Replication during Male Gametogenesis in the Malaria Parasite Plasmodium Falciparum. Cellular Microbiology, 2022. ISSN 1462-5814

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Abstract

Malaria parasites undergo a single phase of sexual reproduction in their complex lifecycle. It involves specialised, sexually committed cells called gametocytes, which develop rapidly into mature gametes and mate upon entering the mosquito midgut. Gamete development is unique, involving unprecedentedly fast replication to produce male gametes. Within ~15 minutes a male gametocyte replicates its ~23 Mb genome three times to produce 8 genomes, segregates these into newly-assembled flagellated gametes and releases them to seek female gametes. Here, for the first time, we use fluorescent labelling of de novo DNA synthesis to follow this process at the whole-cell and single-molecule levels. We make several novel observations, including characterising the origin recognition complex protein Orc1 for the first time in gametocytes, finding that cytokinesis is uncoupled from DNA replication (implying a lack of cell cycle checkpoints), and that the single-molecule dynamics of DNA replication are entirely different from the dynamics in asexual schizogony.

Item Type: Article
Additional Information: Copyright © 2022 Holly Matthews et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Subjects: Q Science > QD Chemistry > QD415 Biochemistry
R Medicine > R Medicine (General) > R735 Medical education. Medical schools. Research
Divisions: Faculty of Natural Sciences > School of Life Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 10 Nov 2022 09:18
Last Modified: 10 Nov 2022 09:18
URI: https://eprints.keele.ac.uk/id/eprint/11660

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