Pollegioni, P, Persampieri, T, Minuz, RL, Bucci, A, Trusso, A, Di Martino, S, Leo, C, Bruttini, M, Ciolfi, M, Waldvogel, A-M, Tripet, F, Simoni, A, Crisanti, A and Müller, R (2022) Introgression of a synthetic sex ratio distortion transgene into different genetic backgrounds of Anopheles coluzzii. Insect Molecular Biology. ISSN 1365-2583

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Abstract

The development of genetically modified mosquitoes (GMM) and their subsequent field release offers innovative approaches for vector control of malaria. A non-gene drive self-limiting male-bias Ag(PMB)1 strain has been developed in a 47-year-old laboratory G3 strain of Anopheles gambiae s.l. When Ag(PMB)1 males are crossed to wild-type females, expression of the endonuclease I-PpoI during spermatogenesis causes the meiotic cleavage of the X chromosome in sperm cells, leading to fertile offspring with a 95% male bias. However, WHO states that the functionality of the transgene could differ when inserted in different genetic backgrounds of An. coluzzii which is currently a predominant species in several West-African countries and thus a likely recipient for a potential release of self-limiting GMMs. In this study, we introgressed the transgene from the donor Ag(PMB)1 by six serial backcrosses into two recipient colonies of An. coluzzii that had been isolated in Mali and Burkina Faso. Scans of informative SNP markers and whole-genome sequencing analysis revealed a nearly complete introgression of chromosome 3 and X, but a remarkable genomic divergence in a large region of the chromosome 2 between the later backcrossed (BC6) transgenic offspring and the recipient paternal strains. These findings suggested to extend the backcrossing breeding strategy beyond BC6 generation and increase the introgression efficiency of critical regions that have ecological and epidemiological implications through the targeted selection of specific markers. Disregarding of differential introgression efficiency, we concluded that the phenotype of the sex ratio distorter is stabile in the BC6 introgressed An. coluzzii strains. This article is protected by copyright. All rights reserved.

Item Type: Article
Additional Information: © 2022 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords: anopheles; genomic divergence; introgression; malaria vector; sex-ratio distorter
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Natural Sciences > School of Life Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 04 Nov 2022 09:35
Last Modified: 04 Nov 2022 09:35
URI: https://eprints.keele.ac.uk/id/eprint/11675

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