Henderson, C, Brustolin, M, Hegde, S, Dayama, G, Lau, N, Hughes, GL, Bergey, C and Rasgon, JL (2022) Transcriptomic and small RNA response to Mayaro virus infection in Anopheles stephensi mosquitoes. PLOS Neglected Tropical Diseases, 16 (6). 1 - 21. ISSN 1935-2727

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<jats:p>Mayaro virus (MAYV) is an arboviral pathogen in the genus <jats:italic>Alphavirus</jats:italic> that is circulating in South America with potential to spread to naïve regions. MAYV is also one of the few viruses with the ability to be transmitted by mosquitoes in the genus <jats:italic>Anopheles</jats:italic>, as well as the typical arboviral transmitting mosquitoes in the genus <jats:italic>Aedes</jats:italic>. Few studies have investigated the infection response of <jats:italic>Anopheles</jats:italic> mosquitoes. In this study we detail the transcriptomic and small RNA responses of <jats:italic>An</jats:italic>. <jats:italic>stephensi</jats:italic> to infection with MAYV via infectious bloodmeal at 2, 7, and 14 days post infection (dpi). 487 unique transcripts were significantly regulated, 78 putative novel miRNAs were identified, and an siRNA response is observed targeting the MAYV genome. Gene ontology analysis of transcripts regulated at each timepoint shows a number of proteases regulated at 2 and 7 dpi, potentially representative of Toll or melanization pathway activation, and repression of pathways related to autophagy and apoptosis at 14 dpi. These findings provide a basic understanding of the infection response of <jats:italic>An</jats:italic>. <jats:italic>stephensi</jats:italic> to MAYV and help to identify host factors which might be useful to target to inhibit viral replication in <jats:italic>Anopheles</jats:italic> mosquitoes.</jats:p>

Item Type: Article
Additional Information: © 2022 Henderson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
S Agriculture > S Agriculture (General)
S Agriculture > SF Animal culture
Depositing User: Symplectic
Date Deposited: 11 Nov 2022 12:07
Last Modified: 11 Nov 2022 12:07
URI: https://eprints.keele.ac.uk/id/eprint/11685

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