Lou, T, Bai, X, He, X, Liu, W, Yang, Z, Yang, Y and Yuan, C (2023) Enhanced antifouling properties of marine antimicrobial peptides by PEGylation. Frontiers in Bioengineering and Biotechnology, 11. 1124389 - ?. ISSN 2296-4185

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Covalent immobilisation of antimicrobial peptides (AMPs) on underwater surfaces to combat marine biofouling is of great interest as it is an efficient, broad-spectrum and environmentally friendly strategy. Similar to post-translational modifications of natural proteins, artificial modifications of antimicrobial peptides can introduce important impacts on their properties and functions. The present work revealed the enhanced effect of PEGylation on the antifouling properties of marine antimicrobial peptides (LWFYTMWH) through grafting the modified peptides on aluminium surfaces. PEG was coupled to the peptide by solid-phase peptide synthesis, and the PEGylated peptides were bioconjugated to the aluminium surfaces which was pre-treated by aryldiazonium salts to introduce carboxyl groups. The carboxy group has been activated through the reaction with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide. The successful modification was confirmed via FT-IR and XPS. Interestingly, the PEGylated peptides modified surfaces could kill 90.0% Escherichia coli (Gram-negative) and 76.1% Bacillus sp. (Gram-positive), and showed better antifouling performance than the original peptides modified surfaces. Furthermore, molecular dynamics simulations showed PEGylation could enhance the ability of peptides to destroy membrane. The PEGylated peptides inserted into the membrane and induced the change in local curvature of membrane, leading to the rupture of membrane. The presence of PEG changed the antimicrobial peptides into more flexible conformations and the high hydrophilicity of PEG hindered the settlement of bacteria. These might be the two main working mechanisms for the increased antifouling efficiency of PEGylated peptides modified surface. This study provided a feasible modification strategy of antimicrobial peptides to enhance their antifouling properties.

Item Type: Article
Additional Information: © 2023 Lou, Bai, He, Liu, Yang, Yang and Yuan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Uncontrolled Keywords: marine biofouling; marine-based peptide; PEGylation; aryldiazonium salts; molecular dynamics simulation; aluminium surface
Subjects: R Medicine > R Medicine (General)
R Medicine > R Medicine (General) > R735 Medical education. Medical schools. Research
Divisions: Faculty of Medicine and Health Sciences > School of Pharmacy and Bioengineering
Related URLs:
Depositing User: Symplectic
Date Deposited: 24 Mar 2023 13:45
Last Modified: 13 Apr 2023 14:48
URI: https://eprints.keele.ac.uk/id/eprint/12067

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