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Miller, GJ, Hansen, SU, Barath, M, Johannessen, C, Blanch, EW, Jayson, GC and Gardiner, JM (2014) Synthesis of a heparin-related GlcN-IdoA sulfation-site variable disaccharide library and analysis by Raman and ROA spectroscopy. Carbohydrate Research, 400. 44 -53. ISSN 0008-6215
Full text not available from this repository.Abstract
Synthesis of an array of differentially sulfated GlcN–IdoA disaccharides, accessible on good scale, directly from l-iduronate components is described. These are specifically directed to provide the sulfation variability at the key most common biologically relevant sulfation-variable l-IdoA O-2 and d-GlcN O-6 and amino sites of this heparin disaccharide. This sulfation-varied matrix has allowed the first evaluation of using Raman/ROA spectroscopy to characterize changes in spectra as a function of both site and level of sulfation with pure, defined heparin-related disaccharide species. This provides analysis of both similarities and differences to digest native heparin and this shows evidence of different types of changes in conformations and conformational freedom as a function of some specific sulfation changes at the disaccharide level. It is anticipated that this data set will open the way for applications to further site-specific sulfated saccharides and demonstrates the capability offered by Raman–ROA towards fingerprinting sulfation in heparin fragments.
Item Type: | Article |
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Uncontrolled Keywords: | Disaccharides, Heparin, Sulfated carbohydrates, Raman–ROA; Iduronic aid |
Subjects: | Q Science > QD Chemistry R Medicine > R Medicine (General) |
Divisions: | Faculty of Natural Sciences > School of Physical and Geographical Sciences |
Related URLs: | |
Depositing User: | Symplectic |
Date Deposited: | 26 Nov 2015 09:21 |
Last Modified: | 08 May 2019 13:55 |
URI: | https://eprints.keele.ac.uk/id/eprint/1227 |