Wilkinson, H, McCarthy, HS, Perry, J, Smith, T, Wright, K and Cool, P (2023) Genomic Sequencing to Diagnose Prosthetic Joint Infection in the Knee: A Case Report. Cureus. ISSN 2168-8184

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There is currently no "gold-standard" method to diagnose prosthetic joint infections (PJI), and the current practice of using microbiological cultures has many limitations. The identification of the bacterial species causing the infection is crucial to guide treatment; therefore, a robust method needs to be developed. Here, we attempt to use genomic sequencing with the MinION device from Oxford Nanopore Technologies to identify the species of bacteria causing PJI in a 61-year-old male. Genomic sequencing with the MinION presents an opportunity to produce species identification in real-time and at a smaller cost than current methods. By comparing results with standard hospital microbiological cultures, this study suggests that nanopore sequencing using the MinION could be a faster and more sensitive method to diagnose PJI than microbiological cultures.

Item Type: Article
Additional Information: Wilkinson H, McCarthy H S, Perry J, et al. (May 09, 2023) Genomic Sequencing to Diagnose Prosthetic Joint Infection in the Knee: A Case Report. Cureus 15(5): e38788. doi:10.7759/cureus.38788
Uncontrolled Keywords: orthopaedics; nanopore; diagnosis; infection; genomics
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > School of Pharmacy and Bioengineering
Depositing User: Symplectic
Date Deposited: 15 May 2023 08:23
Last Modified: 15 May 2023 08:23
URI: https://eprints.keele.ac.uk/id/eprint/12643

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