Alasdair Gawain Kay
BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation.
Gawain Kay, Alasdair; Dale, Tina; Mehedi Akram, Khondoker; Mohan, Param; Hampson, Karen; Maffulli, Nicola; Spiteri, Monica A; El Haj, Alicia; Forsyth, Nicholas
Authors
Tina Dale t.p.dale@keele.ac.uk
Khondoker Mehedi Akram
Param Mohan
Karen Hampson
Nicola Maffulli
Monica A Spiteri
Alicia El Haj
Nicholas Forsyth
Abstract
AIM: Human mesenchymal stem cells (hMSC) are multipotent progenitor cells. We propose the optimization of hMSC isolation and recovery using the application of a controlled hypoxic environment. MATERIALS & METHODS: We evaluated oxygen, glucose and serum in the recovery of hMSC from bone marrow (BMhMSC). Colony forming units-fibroblastic, cell numbers, tri-lineage differentiation, immunofluorescence and microarray were used to confirm and characterize BMhMSC. RESULTS: In an optimized (2% O(2), 4.5 g/l glucose and 5% serum) environment both colony forming units-fibroblastic (p = 0.01) and cell numbers (p = 0.0001) were enhanced over standard conditions. Transcriptional analysis identified differential expression of bone morphogenetic protein 2 (BMP2) and, putatively, chemokine (C-X-C motif) receptor 2 (CXCR2) signaling pathways. CONCLUSION: We have detailed a potential milestone in the process of refinement of the BMhMSC isolation process.
| Journal Article Type | Article |
|---|---|
| Online Publication Date | Apr 2, 2015 |
| Publication Date | 2015-03 |
| Publicly Available Date | May 26, 2023 |
| Journal | Regen Med |
| Print ISSN | 1746-0751 |
| Publisher | Future Medicine |
| Peer Reviewed | Peer Reviewed |
| Volume | 10 |
| Issue | 2 |
| Pages | 109 - 125 |
| DOI | https://doi.org/10.2217/rme.14.67 |
| Keywords | Mesenchymal stem cells, physiological normoxia, hypoxia, BMP2, transcriptome, CFU-F |
| Publisher URL | http://www.futuremedicine.com/doi/10.2217/rme.14.67 |
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