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Lowndes, M, Rotherham, M, Price, J, El Haj, AJ and Habib, S (2016) Immobilized WNT Proteins Act as Stem Cell Niche for Tissue Engineering. Stem Cell Reports, 7 (1). 126 - 137. ISSN 2213-6711

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Abstract

The timing, location, and level of WNT signaling are highly regulated during embryonic development and for the maintenance of adult tissues. Consequently the ability to provide a defined and directed source of WNT proteins is crucial to fully understand its role in tissue development and to mimic its activity in vitro. Here we describe a one-step immobilization technique to covalently bind WNT3A proteins as a basal surface with easy storage and long-lasting activity. We show that this platform is able to maintain adult and embryonic stem cells while also being adaptable for 3D systems. Therefore, this platform could be used for recapitulating specific stem cell niches with the goal of improving tissue engineering.

Item Type: Article
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Depositing User: Symplectic
Date Deposited: 14 Jul 2016 10:45
Last Modified: 10 Apr 2019 11:27
URI: https://eprints.keele.ac.uk/id/eprint/2035

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