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Further development of an in vitro model for studying the penetration of chemicals through compromised skin

Further development of an in vitro model for studying the penetration of chemicals through compromised skin Thumbnail


Abstract

A new in vitro model based on the electrical resistance properties of the skin barrier has been established in this laboratory. The model utilises a tape stripping procedure in dermatomed pig skin that removes a specific proportion of the stratum corneum, mimicking impaired barrier function observed in humans with damaged skin. The skin penetration and distribution of chemicals with differing physicochemical properties, namely; Benzoic acid, 3-Aminophenol, Caffeine and Sucrose has been assessed in this model. Although, skin penetration over 24 h differed for each chemical, compromising the skin did not alter the shape of the time course profile, although absorption into receptor fluid was higher for each chemical. Systemic exposure (receptor fluid, epidermis and dermis), was marginally higher in compromised skin following exposure to the fast penetrant, Benzoic acid, and the slow penetrant Sucrose. The systemically available dose of 3-Aminophenol increased to a greater extent and the absorption of Caffeine was more than double in compromised skin, suggesting that Molecular Weight and Log Pow, are not the only determinants for assessing systemic exposure under these conditions. Although further investigations are required, this in vitro model may be useful for prediction of dermal route exposure under conditions where skin barrier is impaired.

Acceptance Date Oct 11, 2016
Publication Date Oct 14, 2016
Publicly Available Date Mar 28, 2024
Journal Toxicology in Vitro
Print ISSN 0887-2333
Publisher Elsevier
Pages 101-107
DOI https://doi.org/10.1016/j.tiv.2016.10.004
Keywords dermatomed pig skin; electrical resistance; barrier function; compromised skin; tape stripping; in vitro percutaneous absorption
Publisher URL https://doi.org/10.1016/j.tiv.2016.10.004

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