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Development of a nanomaterial bio-screening platform for neurological applications

Jenkins, SI; Roach, P; Chari, DM

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Authors

P Roach



Abstract

Nanoparticle platforms are being intensively investigated for neurological applications. Current biological models used to identify clinically relevant materials have major limitations, e.g. technical/ethical issues with live animal experimentation, failure to replicate neural cell diversity, limited control over cellular stoichiometries and poor reproducibility. High-throughput neuro-mimetic screening systems are required to address these challenges. We describe an advanced multicellular neural model comprising the major non-neuronal/glial cells of the central nervous system (CNS), shown to account for ~99.5% of CNS nanoparticle uptake. This model offers critical advantages for neuro-nanomaterials testing while reducing animal use: one primary source and culture medium for all cell types, standardized biomolecular corona formation and defined/reproducible cellular stoichiometry. Using dynamic time-lapse imaging, we demonstrate in real-time that microglia (neural immune cells) dramatically limit particle uptake in other neural subtypes (paralleling post-mortem observations after nanoparticle injection in vivo), highlighting the utility of the system in predicting neural handling of biomaterials.

Acceptance Date Jul 22, 2014
Publication Date Jan 1, 2015
Publicly Available Date Mar 28, 2024
Journal Nanomedicine: Nanotechnology, Biology and Medicine
Print ISSN 1549-9634
Publisher Elsevier
Pages 77 - 87
DOI https://doi.org/10.1016/j.nano.2014.07.010
Keywords biomaterials screening, glia, multicellular models, neural cells, protein corona, animal use alternatives, animals, astrocytes, biocompatible materials, central nervous system, coculture techniques, culture media, croglia, microscopy, fluorescence, nanome
Publisher URL http://dx.doi.org/10.1016/j.nano.2014.07.010

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