McCarthy, HS, Williams, JHH, Davie, MWJ and Marshall, MJ (2008) Platelet-Derived Growth Factor Stimulates Osteoprotegerin Production in Osteoblastic Cells. Journal of Cellular Physiology, 218 (2). pp. 350-354. ISSN 0021-9541

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Osteoprotegerin (OPG) is a major regulator of osteoclastogenesis, bone resorption and vascular calcification. OPG is produced by various cell types including mesenchymally derived cells, in particular, osteoblastic cells. Here we show OPG production by osteoblastic cells was stimulated by platelet‐derived growth factor (PDGF) in two human osteosarcoma cell lines (MG63, Saos‐2), a mouse pre‐osteoblastic cell line (MC3T3‐E1) and human bone marrow stromal cells (hMSC) by 152%, 197%, 113% and 45% respectively over 24 h. OPG was measured in the cell culture medium by immunoassay. PDGF isoforms AA, BB and AB show similar stimulation of OPG production. Message for OPG was also increased similarly to the increased secretion into the culture medium. Using specific inhibitors of cell signalling we demonstrate that PDGF acts through the PDGF receptor, PKC, PI3K, ERK and P38 and not via NF‐kB or JNK. The importance of PDGF in fracture healing suggests a role for OPG production in countering bone resorption during the early phase of this process.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Depositing User: Symplectic
Date Deposited: 15 Dec 2016 15:47
Last Modified: 16 Jul 2019 08:16

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