Dent, SD, Xia, D, Wastling, JM, Neuman, BW, Britton, P and Maier, HJ (2015) The proteome of the infectious bronchitis virus Beau-R virion. Journal of General Virology, 96 (12). 3499 - 3506. ISSN 1465-2099

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Infectious bronchitis is a highly contagious respiratory disease of poultry caused by the coronavirus infectious bronchitis virus (IBV). It was thought that coronavirus virions were composed of three major viral structural proteins until investigations of other coronaviruses showed that the virions also include viral non-structural and genus-specific accessory proteins as well as host-cell proteins. To study the proteome of IBV virions, virus was grown in embryonated chicken eggs, purified by sucrose-gradient ultracentrifugation and analysed by mass spectrometry. Analysis of three preparations of purified IBV yielded the three expected structural proteins plus 35 additional virion-associated host proteins. The virion-associated host proteins had a diverse range of functional attributions, being involved in cytoskeleton formation, RNA binding and protein folding pathways. Some of these proteins were unique to this study, while others were found to be orthologous to proteins identified in severe acute respiratory syndrome coronavirus virions and also virions from a number of other RNA and DNA viruses.

Item Type: Article
Uncontrolled Keywords: Allantois, Animals, Chick Embryo, Gene Expression Regulation, Viral, Infectious bronchitis virus, Mass Spectrometry, Proteome, Specific Pathogen-Free Organisms, Viral Proteins, Virion

Q Science > QH Natural history

Divisions: Faculty of Natural Sciences > School of Life Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 18 May 2017 08:28
Last Modified: 18 May 2017 08:28

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