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Locally Excited State - Charge Transfer State Coupled Dyes as Optically Responsive Neuron Firing Probes

Abstract

A selection of NIR-optically responsive neuron probes was produced comprising of a donor julolidyl group connected to a BODIPY core and several different styryl and vinylpyridinyl derived acceptor moieties. The strength of the donor–acceptor interaction was systematically modulated by altering the electron withdrawing nature of the aryl unit. The fluorescence quantum yield was observed to decrease as the electron withdrawing effect of the aryl subunit increased in line with changes of the Hammett parameter. The effectiveness of these fluorophores as optically responsive dyes for neuronal imaging was assessed by measuring the toxicity and signal-to-noise ratio (SNR) of each dye. A great improvement of SNR was obtained when compared to the first-generation BODIPY-based voltage sensitive dyes with concomitant toxicity decrease. The mechanism for the optical response is disparate from conventional cyanine-based dyes, opening up a new way to produce effective voltage sensitive dyes that respond well into the NIR region.

Acceptance Date Aug 18, 2017
Publication Date Aug 21, 2017
Publicly Available Date Mar 28, 2024
Journal Chemistry - A European Journal
Print ISSN 0947-6539
Publisher Wiley
Pages 14639-14649
DOI https://doi.org/10.1002/chem.201703366
Keywords BODIPY, neural imaging, fluorescence, voltage sensitive dyes, photochemistry
Publisher URL http://dx.doi.org/10.1002/chem.201703366

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