Wojtas, DH, Ayyer, K, Liang, M, Mossou, E, Romoli, F, Seuring, C, Beyerlein, KR, Bean, RJ, Morgan, AJ, Oberthuer, D, Fleckenstein, H, Heymann, M, Gati, C, Yefanov, O, Barthelmess, M, Ornithopoulou, E, Galli, L, Xavier, PL, Ling, WL, Frank, M, Yoon, CH, White, TA, Bajt, S, Mitraki, A, Boutet, S, Aquila, A, Barty, A, Forsyth, VT, Chapman, HN and Millane, RP (2017) Analysis of XFEL serial diffraction data from individual crystalline fibrils. IUCRJ, 4. 795 -811. ISSN 2052-2525

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Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils; these patterns are weak and contain only a few reflections. Methods are developed for determining the orientation of patterns in reciprocal space and merging them in three dimensions. This allows the individual structure
amplitudes to be calculated, thus overcoming the limitations of orientation and cylindrical averaging in conventional fibre diffraction analysis. The advantages of this technique should allow structural studies of fibrous systems in biology that are inaccessible using existing techniques.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via IUC at http://dx.doi.org/10.1107/S2052252517014324 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: serial crystallography, coherent X-ray diffractive imaging (CXDI), single particles, molecular orientation determination, crystalline fibrils, amyloid
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Natural Sciences > School of Life Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 16 Nov 2017 10:33
Last Modified: 16 Nov 2017 10:35
URI: https://eprints.keele.ac.uk/id/eprint/4233

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