Gillies, D, Gamal, W, Downes, A, Reinwald, Y, Yang, Y, El Haj, AJ and Bagnaninchi, PO (2018) Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy. Methods, 136. pp. 126-133. ISSN 1095-9130

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Cell mechanical behaviour is increasingly recognised as a central biophysical parameter in cancer and stem cell research, and methods of investigating their mechanical behaviour are therefore needed. We have developed a novel qualitative method based on quantitative phase imaging which is capable of investigating cell mechanical behaviour in real-time at cellular resolution using optical coherence phase microscopy (OCPM), and stimulating the cells non-invasively using hydrostatic pressure. The method was exemplified to distinguish between cells with distinct mechanical properties, and transient change induced by Cytochalasin D. We showed the potential of quantitative phase imaging to detect nanoscale intracellular displacement induced by varying hydrostatic pressure in microfluidic channels, reflecting cell mechanical behaviour. Further physical modelling is required to yield quantitative mechanical properties.

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via Elsevier at - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: optical coherence phase microscopy, mechanical behaviour, real-time monitoring, hydrostatic pressure, phase imaging
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 16 Jan 2018 16:14
Last Modified: 26 Jan 2021 16:43

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