Kalha, S, Shrestha, B, Sanz Navarro, M, Jones, KB, Klein, OD and Michon, F (2018) Bmi1+ Progenitor Cell Dynamics in Murine Cornea During Homeostasis and Wound Healing. Stem Cells, 36 (4). pp. 562-573. ISSN 1549-4918

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The outermost layer of the eye, the cornea, is renewed continuously throughout life. Stem cells of the corneal epithelium reside in the limbus at the corneal periphery and ensure homeostasis of the central epithelium. However, in young mice, homeostasis relies on cells located in the basal layer of the central corneal epithelium. Here, we first studied corneal growth during the transition from newborn to adult and assessed Keratin 19 (Krt19) expression as a hallmark of corneal maturation. Next, we set out to identify a novel marker of murine corneal epithelial progenitor cells before, during and after maturation, and we found that Bmi1 is expressed in the basal epithelium of the central cornea and limbus. Furthermore, we demonstrated that Bmi1+ cells participated in tissue replenishment in the central cornea. These Bmi1+ cells did not maintain homeostasis of the cornea for more than 3 months, reflecting their status as progenitor rather than stem cells. Finally, after injury, Bmi1+ cells fueled homeostatic maintenance, whereas wound closure occurred via epithelial reorganization. Stem Cells 2018.

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via AlphaMed Press at http://doi.org/10.1002/stem.2767 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: Bmi1, cornea, maturation, progenitor, renewal, stem cell, wound healing
Subjects: R Medicine > R Medicine (General)
R Medicine > RA Public aspects of medicine
Divisions: Faculty of Medicine and Health Sciences > Primary Care Health Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 14 Mar 2018 15:34
Last Modified: 28 Jun 2018 01:30
URI: https://eprints.keele.ac.uk/id/eprint/4569

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