S. Roberts
ADAMTS-4 activity in synovial fluid as a biomarker of inflammation and effusion
Roberts, S.; Evans, H.; Wright, K.; van Niekerk, L.; Caterson, B.; Richardson, J.B.; Kumar, K.H.S.; Kuiper, J.H.
Authors
H. Evans
Karina Wright k.t.wright@keele.ac.uk
L. van Niekerk
B. Caterson
J.B. Richardson
K.H.S. Kumar
Jan Kuiper j.h.kuiper@keele.ac.uk
Abstract
Objective
To evaluate the potential of ADAMTS-4 (aggrecanase -1) activity in synovial fluid (SF) as a biomarker of knee injury and joint disease.
Design
We have measured ADAMTS-4 activity in the synovial fluid of 170 orthopaedic patients with different degrees of joint pathology, using a commercial ADAMTS-4 fluorescence resonance energy transfer (FRET) substrate assay. Patients were classified at arthroscopy as (i) macroscopically normal, (ii) with an injury of the meniscus, anterior cruciate ligament or chondral/osteochondral defects or (iii) with osteoarthritis, and the influence of independent factors (age, patient group, effusion and synovial inflammation) on ADAMTS-4 activity levels was assessed.
Results
In most patients (106/170) ADAMTS-4 activity was undetectable; ADAMTS-4 ranged from 0 to 2.8 ng/mL in synovial fluid from patients with an injury, 0–4.1 ng/mL in osteoarthritic patients and 4.0–12.3 ng/mL in patients with large effusions. Four independent variables each significantly influenced ADAMTS-4 activity in synovial fluid (all P < 0.001): age (concordance = 0.69), presence of osteoarthritis (OA) (concordance = 0.66), level of effusion (concordance = 0.78) and inflammation (concordance = 0.68). Not only did effusion influence the amount of ADAMTS-4 activity most strongly, but it also did this in an ordered manner (P < 0.001).
Conclusions
The main finding of this study is that ADAMTS-4 levels in synovial fluid are most strongly correlated with inflammation and severity of effusion in the knee. Further study is required to determine if it could provide a useful tool to aid clinical diagnoses, indicate treatment, to monitor progression of joint degeneration or OA or alternatively the success of treatment.
Journal Article Type | Article |
---|---|
Acceptance Date | May 12, 2015 |
Online Publication Date | May 21, 2015 |
Publication Date | Sep 1, 2015 |
Publicly Available Date | May 26, 2023 |
Journal | Osteoarthritis and Cartilage |
Print ISSN | 1063-4584 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 23 |
Issue | 9 |
Pages | 1622-1626 |
DOI | https://doi.org/10.1016/j.joca.2015.05.006. |
Keywords | ADAMTS-4 activity, Synovial fluid, Inflammation, Effusion, Knee osteoarthritis |
Publisher URL | https://doi.org/10.1016/j.joca.2015.05.006 |
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