Gilchrist, FJ, Spanel, P, Smith, D and Lenney, W (2015) The in vitro identification and quantification of volatile biomarkers released by cystic fibrosis pathogens. Analytical Methods, 7 (3). 818 -824. ISSN 1759-9660

[thumbnail of c4ay02981j.pdf]
c4ay02981j.pdf - Published Version
Available under License Creative Commons Attribution.

Download (803kB) | Preview


There is interest in the development of exhaled breath tests for the detection of lower airway infection in children with cystic fibrosis. The first stage of this process is the identification of volatile organic compounds (VOCs) released into the gas phase by CF pathogens that can be used as breath test biomarkers. Selected ion flow tube mass spectrometry (SIFT-MS) is ideally suited to these in vitro studies as it allows simultaneous quantification of multiple VOCs in real time. We review a decade of in vitro experiments using SIFT-MS to analyse the VOCs released by respiratory pathogens. This includes identification and quantification of VOCs and the investigation of the in vitro factors that affect their production. We also report on how our culture methodology has been refined over the years to better account for variations in bacterial mass. Finally, we discuss how these in vitro findings have been translated into clinical trials and assess possible future applications.

Item Type: Article
Uncontrolled Keywords: breath analysis, cystic fibrosis, biomarkers
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 25 May 2015 09:13
Last Modified: 09 May 2019 14:32

Actions (login required)

View Item
View Item