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Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices

Adams, CF; Delaney, AM; Carwardine, DR; Tickle, J; Granger, N; Chari, DM

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Authors

AM Delaney

DR Carwardine

J Tickle

N Granger



Abstract

A recent clinical trial proves that autologous olfactory mucosal cell (OMC) transplantation improves locomotion in dogs with naturally occurring spinal injuries comparable to human lesions. However, not all dogs respond to the treatment, likely due to the transplantation procedures involving injections of cell suspensions that are associated with cell death, uneven cell distribution, and cell washout. Encapsulating cells in protective hydrogel matrices offers a tissue engineering solution to safely achieve 3D growth of viable transplant cells for implantation into injury sites, to improve regenerative outcomes. It is shown for the first time that canine OMCs (cOMCs) can be propagated with high viability in 3D collagen matrices. Further, a method to incorporate cOMCs pre-labeled with clinical-grade iron oxide nanoparticles into the constructs is described. Intraconstruct labeled cells are visualized using magnetic resonance imaging, offering substantial promise for in vivo tracking of cOMCs delivered in protective matrices.

Journal Article Type Article
Acceptance Date Dec 4, 2018
Publication Date Feb 1, 2019
Publicly Available Date Mar 28, 2024
Journal Macromolecular Bioscience
Print ISSN 1616-5187
Electronic ISSN 1616-5195
Publisher Wiley
Volume 19
Pages e1800389
DOI https://doi.org/10.1002/mabi.201800389
Keywords canine olfactory mucosal cell, cell transplantation, hydrogel, magnetic nanoparticle, spinal injury, Animals, Cell- and Tissue-Based Therapy, Cells, Cultured, Collagen, Dogs, Hydrogels, Magnetic Resonance Imaging, Magnetite Nanoparticles, Models, Animal,
Publisher URL https://www.ncbi.nlm.nih.gov/pubmed/30511815

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