Holmes, AM, Heylings, JR, Wan, K-W and Moss, GPJ (2019) Antimicrobial efficacy and mechanism of action of poly(amidoamine) (PAMAM) dendrimers against opportunistic pathogens. International Journal of Antimicrobial Agents, 53 (4). pp. 500-507. ISSN 1872-7913

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The aim of this study was to investigate a range of poly(amidoamine) (PAMAM) dendrimer generations against Gram-positive and Gram-negative skin pathogens and to determine any differences in antimicrobial potency for different generations, characterising how differences in physicochemical properties influence antimicrobial efficacy. A range of tests were carried out, including viable count assays to determine IC50 values for each dendrimer, membrane integrity studies and an inner membrane permeabilisation assay. This is supported by scanning electron microscopy imaging of the interactions observed between dendrimers and bacteria. The results of this study indicate that the antimicrobial efficacy of native PAMAM dendrimers is dependent on generation, concentration and terminal functionalities, for example the MIC50 (υg/mL) against S. aureus was between 26.77 for the G2-PAMAM-NH2 dendrimer and 2.881 for the G5-PAMAM-NH2 dendrimer. There was a strong correlation between membrane disruption and the determined biocidal activity, making it a key contributing mechanism of action. This study demonstrates that selection of the type of PAMAM dendrimer is important as their inherent antimicrobial efficacy varies according to their individual physicochemical properties. This understanding may pave the way for the development of enhanced dendrimer-based antimicrobial formulations and drug delivery systems.

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via Elsevier at https://doi.org/10.1016/j.ijantimicag.2018.12.012 - Please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: Antimicrobial, Biocide, Novel antiseptic, Polyamidoamine dendrimers, Skin
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Faculty of Medicine and Health Sciences > School of Pharmacy
Related URLs:
Depositing User: Symplectic
Date Deposited: 07 Jan 2019 10:39
Last Modified: 30 Dec 2019 01:30
URI: https://eprints.keele.ac.uk/id/eprint/5635

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