Helling, AL, Viswanathan, P, Cheliotis, KS, Mobasseri, SA, Yang, Y, El Haj, AJ and Watt, FM (2019) Dynamic Culture Substrates That Mimic the Topography of the Epidermal-Dermal Junction. Tissue Engineering Part A, 25 (3-4). 214 - 223. ISSN 1937-335X

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The junction between the epidermal and dermal layers of human skin undulates, the width and depth of the undulations varying with age and disease. We previously showed that when primary human epidermal keratinocytes are seeded on collagen-coated undulating static polydimethylsiloxane (PDMS) elastomer substrates, the stem cells, differentiated cells, and proliferating cells become patterned in response to cues from the underlying substrate. To investigate how patterning occurs over time, we have now created a dynamic model, in which a collagen-coated poly(d,l-lactide-co-glycolide) (PLGA) membrane is placed over a polyimide sheet containing circular holes, differing in diameter and spacing. When a vacuum is applied the membrane is induced to undulate, the heights of the undulations depending on the pressure applied and the size of the holes. We observed clustering of cells with high levels of b1 integrin expression, a stem cell marker, in the base of the undulations within 48 h of applying the vacuum. Differentiating involucrin-positive cells did not cluster; however, there was clustering of cells with high E-cadherin expression and nuclear YAP. Rho kinase inhibition resulted in loss of clustering, suggesting a role for Rho family members in the process.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via Mary Ann Liebert at https://doi.org/10.1089/ten.tea.2018.0125 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: human epidermal–dermal junction, dynamic substrate, keratinocytes
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 26 Apr 2019 15:20
Last Modified: 26 Jan 2021 16:37
URI: https://eprints.keele.ac.uk/id/eprint/6221

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